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mouse anti smarcal1  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology mouse anti smarcal1
    Mouse Anti Smarcal1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 63 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/smarcal1/SmarcAL1+Antibody/pm41866377-381-5-9
    Average 94 stars, based on 63 article reviews
    mouse anti smarcal1 - by Bioz Stars, 2026-10
    94/100 stars

    Images

    Related Articles

    other:

    Article Title: The DNA replication checkpoint prevents PCNA/RFC depletion to protect forks from HLTF-induced collapse in human cells.
    Article Snippet: Blots were imaged using the LI-COR platform (LI-COR Biosciences) with anti-rabbit RDye 800CW and anti-mouse IRDye680RD secondary antibodies (both 1:10000).


    Article Title: The DNA replication checkpoint prevents PCNA/RFC depletion to protect forks from HLTF-induced collapse in human cells.
    Article Snippet: To quantify the amount of proteins in the insoluble/chromatin fraction, cells were treated with cytoskeleton buffer (CSK) 0.25% Triton X-100 for 5 min on ice.

    Proximity Ligation Assay:

    Article Title: The Ski2 helicase ASCC3 unwinds DNA upon fork stalling to control replication stress responses
    Article Snippet: Drugs used were aphidicolin (Sigma); camptothecin (Sigma); hydroxyurea (BioShop Canada); RAD52 inhibitor (AICAR, Cayman Chemical); REV1 inhibitor JH-RE-06 (SML2993, Sigma); RO-3306 (Selleck Chemicals). .. Antibodies used include: ASCC2 (1:1000; A304-020A, Bethyl Laboratories); ASCC2 (1:5000; 1152-1-AP, Proteintech); ASCC3 (1:400; 17627-1-AP, Proteintech); ASCC3 (1:1000; PA5-56794, Invitrogen); Biotin (1:100000; A150-109A, Bethyl Laboratory); Biotin (1:100000; 200-002-211, Jackson ImmunoResearch); BrdU (1:100; 347580, BD Biosciences); BrdU (1:500; MAB3222, Millipore); BrdU (BU1/75 [ICR1]) (1:800; NB500-169, Novus Biologicals); 53BP1 (1:2000; 612522, BD Biosciences); BRCA1 (1:5000; 07-434, Millipore); BRCA2 (1:2000; 29450-1-AP, Proteintech); CHK1 (1:250; sc-7898, Santa cruz); CHK1-pS345 (1:1000; 2348S, Cell Signaling); CSB (1:200; ab66598, Abcam); FASN (1:20000; 10624-2-AP, Proteintech); GFP (1:1000; 50430-2-AP, Proteintech); HA (1:500; #2367, Cell Signaling); HLTF (1:1000; A300-229A, Bethyl Laboratory); Myc (1:1000; 9E10, Calbiochem); PCNA (1:2000; sc-56, Santa Cruz); PCNA (1:600 for PLA or 1:4000 for western blot; 10205-2-AP, Proteintech); Ubiquityl-PCNA (K164) (1:1000; 13439T, Cell Signaling); PRIMPOL (1:1000; 29824-1-AP, Proteintech); RAD51 (1:2000, ab63801, Abcam); RFWD3 (1:1000; 19893-1-AP, Proteintech); RPA32 (1:10000; NB100-332, Novus Biologicals); RPA32 (1:200; ab2175, Abcam); RPA32-pS33 (1:50000; A300-246A, Bethyl Laboratories); RPA70 (1:2000; 2267S, Cell Signaling); SHPRH (21995-1-AP, Proteintech); SMARCAL1 (1:100, sc-166209, Santa cruz); SMARCAL1 (1:2000; GTX109468, GenTex); TRIP4 (1:1000; 12324-1-AP, Proteintech); α-tubulin (1:20,000; T9026, Sigma); γ-tubulin (1:20,000; GTU88, Sigma). ..

    Western Blot:

    Article Title: The Ski2 helicase ASCC3 unwinds DNA upon fork stalling to control replication stress responses
    Article Snippet: Drugs used were aphidicolin (Sigma); camptothecin (Sigma); hydroxyurea (BioShop Canada); RAD52 inhibitor (AICAR, Cayman Chemical); REV1 inhibitor JH-RE-06 (SML2993, Sigma); RO-3306 (Selleck Chemicals). .. Antibodies used include: ASCC2 (1:1000; A304-020A, Bethyl Laboratories); ASCC2 (1:5000; 1152-1-AP, Proteintech); ASCC3 (1:400; 17627-1-AP, Proteintech); ASCC3 (1:1000; PA5-56794, Invitrogen); Biotin (1:100000; A150-109A, Bethyl Laboratory); Biotin (1:100000; 200-002-211, Jackson ImmunoResearch); BrdU (1:100; 347580, BD Biosciences); BrdU (1:500; MAB3222, Millipore); BrdU (BU1/75 [ICR1]) (1:800; NB500-169, Novus Biologicals); 53BP1 (1:2000; 612522, BD Biosciences); BRCA1 (1:5000; 07-434, Millipore); BRCA2 (1:2000; 29450-1-AP, Proteintech); CHK1 (1:250; sc-7898, Santa cruz); CHK1-pS345 (1:1000; 2348S, Cell Signaling); CSB (1:200; ab66598, Abcam); FASN (1:20000; 10624-2-AP, Proteintech); GFP (1:1000; 50430-2-AP, Proteintech); HA (1:500; #2367, Cell Signaling); HLTF (1:1000; A300-229A, Bethyl Laboratory); Myc (1:1000; 9E10, Calbiochem); PCNA (1:2000; sc-56, Santa Cruz); PCNA (1:600 for PLA or 1:4000 for western blot; 10205-2-AP, Proteintech); Ubiquityl-PCNA (K164) (1:1000; 13439T, Cell Signaling); PRIMPOL (1:1000; 29824-1-AP, Proteintech); RAD51 (1:2000, ab63801, Abcam); RFWD3 (1:1000; 19893-1-AP, Proteintech); RPA32 (1:10000; NB100-332, Novus Biologicals); RPA32 (1:200; ab2175, Abcam); RPA32-pS33 (1:50000; A300-246A, Bethyl Laboratories); RPA70 (1:2000; 2267S, Cell Signaling); SHPRH (21995-1-AP, Proteintech); SMARCAL1 (1:100, sc-166209, Santa cruz); SMARCAL1 (1:2000; GTX109468, GenTex); TRIP4 (1:1000; 12324-1-AP, Proteintech); α-tubulin (1:20,000; T9026, Sigma); γ-tubulin (1:20,000; GTU88, Sigma). ..

    Article Title: A nuclease-driven mechanism of post-replicative ssDNA gap suppression
    Article Snippet: DNA fibres (150 per condition) were visualized on a Zeiss LSM710 confocal microscope, and images were collected using Zen software and then analyzed with ImageJ. .. The following antibodies were used: Western blotting: PRIMPOL (Proteintech: 29824-1-AP, 1:1000), RAD18 (Cell Signalling, D2B8, 1:1000), UBC13 Antibody (Santa Cruz, F-10, 1:1000), GAPDH Antibody (Santa Cruz, G-9, 1:2000), MRNIP Antibody (Santa Cruz, H-11, 1:500), phospho-S217 (in-house, generated by Eurogentec, 1:1000), Tubulin (Abcam, ab4074), SMARCAL1 (Santa Cruz Biotechnology, sc-376377), γH2AX (Cell Signaling Technology, 9718), PRIMPOL (gift from Mendez laboratory, 1:1000). .. Immunofluorescence: 53BP1 (Abcam, ab21083, 1:2000), IdU (BD, 3D4, 1:250) and γH2AX (Upstate, 1:1000).

    Generated:

    Article Title: A nuclease-driven mechanism of post-replicative ssDNA gap suppression
    Article Snippet: DNA fibres (150 per condition) were visualized on a Zeiss LSM710 confocal microscope, and images were collected using Zen software and then analyzed with ImageJ. .. The following antibodies were used: Western blotting: PRIMPOL (Proteintech: 29824-1-AP, 1:1000), RAD18 (Cell Signalling, D2B8, 1:1000), UBC13 Antibody (Santa Cruz, F-10, 1:1000), GAPDH Antibody (Santa Cruz, G-9, 1:2000), MRNIP Antibody (Santa Cruz, H-11, 1:500), phospho-S217 (in-house, generated by Eurogentec, 1:1000), Tubulin (Abcam, ab4074), SMARCAL1 (Santa Cruz Biotechnology, sc-376377), γH2AX (Cell Signaling Technology, 9718), PRIMPOL (gift from Mendez laboratory, 1:1000). .. Immunofluorescence: 53BP1 (Abcam, ab21083, 1:2000), IdU (BD, 3D4, 1:250) and γH2AX (Upstate, 1:1000).



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